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Image Search Results
Journal:
Article Title:
doi:
Figure Lengend Snippet: Mitochondria-associated protein levels in the plantaris muscle. Protein levels of peroxisome proliferator-activated receptor γ coactivator 1-α (PGC-1α) ( A ), NADH:ubiquinone oxidoreductase subunit B8 (NDUFB8) ( B ), succinate dehydrogenase complex iron sulfur subunit B (SDHB) ( C ), ubiquinol–cytochrome c reductase core protein 2 (UQCRC2) ( D ), mitochondrially encoded cytochrome c oxidase 1 (MTCO1) ( E ), cytochrome c oxidase subunit 4I1 (COX4I1/COXIV) ( F ), and ATP synthase F1 subunit alpha (ATP5F1A/ATP5A) ( G ) in the plantaris muscle. Data are expressed as the mean ± SEM ( n = 8–11). Two-way ANOVA was performed to determine the interactions and main effects of training and ORX. ** p < 0.01, * p < 0.05: main effect of training. †† p < 0.01, (†) p ≤ 0.10: main effect of ORX
Article Snippet: Commercially available primary antibodies were used to detect hypoxia-inducible factor 1-α (HIF-1α; #20960-1-AP; Proteintech Japan, Tokyo, Japan), peroxisome proliferator-activated receptor γ coactivator 1-α (PGC-1α; #516557; Merck Millipore), mitochondrial electron transport proteins (NADH:ubiquinone oxidoreductase subunit B8 [NDUFB8], succinate dehydrogenase complex iron sulfur subunit B [SDHB], ubiquinol–cytochrome c reductase core protein 2 [UQCRC2], mitochondrially encoded
Techniques:
Journal:
Article Title:
doi:
Figure Lengend Snippet: Mitochondria-associated protein levels in the soleus muscle. Protein levels of PGC-1α ( A ), NDUFB8 ( B ), SDHB ( C ), UQCRC2 ( D ), MTCO1 ( E ), COXIV ( F ), and ATP5A ( G ) in the soleus muscle. Data are expressed as the mean ± SEM ( n = 8–11). Two-way ANOVA was performed to determine the interactions and main effects of training and ORX. ** p < 0.01, ( * ) p ≤ 0.10: main effect of training. (†) p ≤ 0.10: main effect of ORX
Article Snippet: Commercially available primary antibodies were used to detect hypoxia-inducible factor 1-α (HIF-1α; #20960-1-AP; Proteintech Japan, Tokyo, Japan), peroxisome proliferator-activated receptor γ coactivator 1-α (PGC-1α; #516557; Merck Millipore), mitochondrial electron transport proteins (NADH:ubiquinone oxidoreductase subunit B8 [NDUFB8], succinate dehydrogenase complex iron sulfur subunit B [SDHB], ubiquinol–cytochrome c reductase core protein 2 [UQCRC2], mitochondrially encoded
Techniques:
Journal: iScience
Article Title: Metabolic rewiring and autophagy inhibition correct lysosomal storage disease in mucopolysaccharidosis IIIB
doi: 10.1016/j.isci.2024.108959
Figure Lengend Snippet: Mitochondrial network show signs of disorganization in striatal (upper panels) and cortical (lower panels) regions of NAGLU −/− mice Forty μm-thick coronal brain sections of 8-month-old wild type (WT) and NAGLU −/− mice were stained with the mitochondrial marker mitochondrially encoded cytochrome c oxidase I (MT-CO1, cytochrome c oxidase subunit 1 COX1). Nuclei were stained with Hoechst.
Article Snippet: For Western blot analysis, mouse anti-activated diphosphorylated ERK1/2 monoclonal antibody (M8159) was purchased from Sigma Aldrich Chemical Co., rabbit anti-ERK1/2 polyclonal antibody (V1141) from Promega (USA), mouse anti-phosphorylated Akt monoclonal antibody (sc-514032) was purchased from Santa Cruz Biotechnology (Heidelberg, Germany), mouse anti-Akt polyclonal antibody (sc-5298) Santa Cruz Biotechnology (Heidelberg, Germany), rabbit anti-BCN1 monoclonal antibody (H-300 sc-11427) was purchased from Santa Cruz Biotechnology (Heidelberg, Germany), rabbit anti-LC3 polyclonal antibody (NB100-2331) from Novus Biologicals (USA), mouse anti-GAPDH monoclonal antibody (6C5 sc-32233) from Santa Cruz Biotechnology (Heidelberg, Germany),
Techniques: Staining, Marker
Journal: iScience
Article Title: Metabolic rewiring and autophagy inhibition correct lysosomal storage disease in mucopolysaccharidosis IIIB
doi: 10.1016/j.isci.2024.108959
Figure Lengend Snippet:
Article Snippet: For Western blot analysis, mouse anti-activated diphosphorylated ERK1/2 monoclonal antibody (M8159) was purchased from Sigma Aldrich Chemical Co., rabbit anti-ERK1/2 polyclonal antibody (V1141) from Promega (USA), mouse anti-phosphorylated Akt monoclonal antibody (sc-514032) was purchased from Santa Cruz Biotechnology (Heidelberg, Germany), mouse anti-Akt polyclonal antibody (sc-5298) Santa Cruz Biotechnology (Heidelberg, Germany), rabbit anti-BCN1 monoclonal antibody (H-300 sc-11427) was purchased from Santa Cruz Biotechnology (Heidelberg, Germany), rabbit anti-LC3 polyclonal antibody (NB100-2331) from Novus Biologicals (USA), mouse anti-GAPDH monoclonal antibody (6C5 sc-32233) from Santa Cruz Biotechnology (Heidelberg, Germany),
Techniques: Recombinant, Modification, Protease Inhibitor, Expressing, Clone Assay, Control, Generated, Knock-Out, Software